ImageJ macro for semi-automated counting of nuclei

Published: 20-08-2019| Version 1 | DOI: 10.17632/9fsh4w2s5x.1
Kevin Trout


This semi-automated nuclei counting method gives the user an opportunity to qualitatively review output results for accuracy. The method was designed to work with fluorescently stained nuclei. If desired, the macro could be modified or applied to other staining methods. Although there are a variety of possible methods to count nuclei (e.g. machine learning), the goal of the method presented here was to run quickly and accurately on a basic consumer-level computer with freely available software (ImageJ).