ImageJ macro for semi-automated counting of nuclei

Published: 20 Aug 2019 | Version 1 | DOI: 10.17632/9fsh4w2s5x.1

Description of this data

This semi-automated nuclei counting method gives the user an opportunity to qualitatively review output results for accuracy. The method was designed to work with fluorescently stained nuclei. If desired, the macro could be modified or applied to other staining methods. Although there are a variety of possible methods to count nuclei (e.g. machine learning), the goal of the method presented here was to run quickly and accurately on a basic consumer-level computer with freely available software (ImageJ).

Experiment data files

This data is associated with the following publication:

Factors influencing multinucleated giant cell formation in vitro

Published in: Immunobiology

Latest version

  • Version 1


    Published: 2019-08-20

    DOI: 10.17632/9fsh4w2s5x.1

    Cite this dataset

    Trout, Kevin (2019), “ImageJ macro for semi-automated counting of nuclei”, Mendeley Data, v1


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Downloads: 11


Cell Biology


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The files associated with this dataset are licensed under a Attribution-NonCommercial 3.0 Unported licence.

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You are free to adapt, copy or redistribute the material, providing you attribute appropriately and do not use the material for commercial purposes.