The CCR6–CCL20 axis promotes regulatory T cell glycolysis and immunosuppression in tumors
Description
Data accompanying the manuscript "The CCR6–CCL20 axis promotes regulatory T cell glycolysis and immunosuppression in tumors"-- includes RNA-Seq of sorted murine Tregs from WT and Ccr6 KO mice. The experiment was designed to compare transcriptomic differences between WT and Ccr6 KO Tregs during activation. WT and Ccr6 KO Tregs, cells were isolated from mice and cultured in vitro for 3 days with activation using anti-CD3/CD28 beads. Total RNA was extracted using the Trizol method. Quantity and quality were assessed using a Thermo Scientific™ NanoDrop™ 2000/2000c Spectrophotometer. Novogene Corporation Inc prepared the RNA-seq 250-300 bp insert cDNA library. Illumina HiSeq platform PE150 sequencing was used for sequencing, yielding 20M raw reads/sample. Mus Musculus mm39 was used as the reference genome for alignment.
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WT1, WT2, WT3 = WT Tregs activated for 3 days in vitro with anti-CD3/CD28 antibodies (n = 3) KO1, KO2, KO3 = Ccr6 KO Tregs activated for 3 days in vitro with anti-CD3/CD28 antibodies (n = 3)