Data for: Selection and Validation of Reference Genes for Quantitative real-time PCR in Rosmarinus officinalis in various tissues and under elicitation
Published: 17 July 2019| Version 1 | DOI: 10.17632/9g5j49rygw.1
Contributors:
Mohammad Hossein Mirjalili, Babak Rabiei, Zahra Aminfar, Masoud TohidfarDescription
Fig. S1. Melt curves associated with each of reference gene; gene-specific amplifications is confirmed by the presence of a single peak. (A) Melt curve for 18S rRNA and α-TUB genes; (B) Melt curve for β-TUB and EF1-a genes; (C) Melt curve for GAPDH and CYP genes; (D) Melt curve for ACT gene. The melt curves of each gene are related to three technical replicates. Fig. S2. The specificity of primer pairs for Q-PCR amplification. Agarose gel (1.5%) electrophoresis showing PCR amplified products of the expected size for seven candidate reference genes. M: 1kb marker.
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Gel Electrophoresis, Real-Time Polymerase Chain Reaction