Human co-transcriptional splicing kinetics and coordination revealed by direct nascent RNA sequencing Drexler et al.
Raw images supporting the paper "Human co-transcriptional splicing kinetics and coordination revealed by direct nascent RNA sequencing". In this manuscript, we describe nanopore analysis of CO-transcriptional Processing (nano-COP), a technique designed to directly probe the dynamics and regulation of pre-mRNA splicing in vivo. nano-COP uses direct RNA nanopore sequencing to determine the native isoform of long nascent RNA molecules without amplification-associated biases. Images in Figure 3 are from RT-PCR gels of transcripts from cytoplasm and chromatin fractions from human K562 and Drosophila S2 cells treated with DMSO or varying concentrations of the splicing inhibitor pladienolide B. The genes in the paper (BRD2 for human cells; Set for Drosophila cells) are in the top left quadrant of each figure. Images in Supplemental Figure 1 are from western blots of cellular fractions from Human K562 cells with antibodies against GAPDH and Ser2P Pol II. The data in the manuscript is from the blots on the left side of both images.