Human Brain Small Extracellular Vesicles Contain Selectively-Packaged, Full-Length mRNA

Published: 2 April 2024| Version 1 | DOI: 10.17632/9j94cwxx28.1
Linnea Ransom


Small extracellular vesicles (sEVs) were isolated from human postmortem brain tissue from 12 Alzheimer's disease and 12 non-disease brains using a sucrose density gradient ultracentrifugation protocol adapted from Vella et al 2017. sEVs were RNAase treated to digest extravesicular RNAs. RNA-Seq libraries were generated using poly-A selection and sequenced on Illumina HiSeq and PacBio Sequel II. Raw sequencing data uploaded to European Genome-phenome archive under accession number EGAS50000000029. These data are controlled-access and require a signed data use agreement between the data access committee (DAC) and the user. Small extracellular vesicles were also collected from homogenous cultures of murine primary neurons, microglia, or astrocytes. Poly-A selection was again used for cDNA library preparation. Libraries were sequenced on Illumina NovaSeq and PacBio Sequel II. Raw data is available from GEO under accession numbers GSE243611 and GSE255462.



Sanford Burnham Prebys Medical Discovery Institute


Microglia, Neuron, RNA Sequencing, Brain, Astrocyte, Extracellular Vesicle