Human Brain Small Extracellular Vesicles Contain Selectively-Packaged, Full-Length mRNA
Description
Small extracellular vesicles (sEVs) were isolated from human postmortem brain tissue from 12 Alzheimer's disease and 12 non-disease brains using a sucrose density gradient ultracentrifugation protocol adapted from Vella et al 2017. sEVs were RNAase treated to digest extravesicular RNAs. RNA-Seq libraries were generated using poly-A selection and sequenced on Illumina HiSeq and PacBio Sequel II. Raw sequencing data uploaded to European Genome-phenome archive under accession number EGAS50000000029. These data are controlled-access and require a signed data use agreement between the data access committee (DAC) and the user. Small extracellular vesicles were also collected from homogenous cultures of murine primary neurons, microglia, or astrocytes. Poly-A selection was again used for cDNA library preparation. Libraries were sequenced on Illumina NovaSeq and PacBio Sequel II. Raw data is available from GEO under accession numbers GSE243611 and GSE255462.