Molecular controls over corticospinal neuron segmental target specificity. Sahni et al., Macklis
Description
We FACS purified corticospinal neurons (CSN) in lateral versus medial cortex and analyzed genes differentially expressed between these subpopulations at three distinct developmental times - postnatal day 1 (P1), P4, and P7. The dataset was compiled using three biological replicates at P1, and two biological replicates at P4, and P7. The original microarray dataset is presented here along with gene lists of the top significantly differentially expressed genes in each subpopulation at P1, and P4. The excel files include the following: 1. The complete dataset showing gene intensities (normalized using rosetta resolver) comprising all probes, the normalized intensity for each probe in each biological dataset, the combined average intensity in each subpopulation at each time point, the fold change (of lateral versus medial CSN) at each time point, and the associated p value. 2. The top genes differentially expressed by lateral CSN at P1, ranked by SAM-RMA, along with the normalized rosetta intensities as described above. 3. The top genes differentially expressed by medial CSN at P1, ranked by SAM-RMA, along with the normalized rosetta intensities as described above. 4. The top genes differentially expressed by lateral CSN at P4, ranked by SAM-RMA, along with the normalized rosetta intensities as described above. 5. The top genes differentially expressed by medial CSN at P4, ranked by SAM-RMA, along with the normalized rosetta intensities as described above. The original microarray data has been deposited at the Gene Expression Omnibus database at NCBI (Accession GSE77311).