Palaemon serratus metabolome

Published: 8 September 2022| Version 1 | DOI: 10.17632/9khwhjxyym.1
Benjamin Marie


The prawn Palaemon serratus exhibits a large distribution (occurring along the Northeastern Atlantic coast to the Mediterranean), but presents a small-scale commercial importance, and has thus been found suitable as model organism valuable for various ecotoxicological studies. However, little is still known about the potential input of its metabolome and particularly concerning a potential molecular sexual dimorphism observable in the different tissues of this organism. In an ecotoxicological point of view, inter-sex and inter-organ differences in the metabolome composition might induce bias and influence the robustness of data analysis or interpretation. To explore such possibilities, we obtained qualitative metabolomic data from the analysis of different organs of mature male and female Palaemon serratus. We used ultra-high-performance liquid chromatography-electrospray ionization-high resolution tandem mass spectrometry (UHPLC-ESI-HRMS on positive mode) to characterize the 75%-extracted metabolome of both gills, hepatopancreas, nervous gland, muscle and gonads. The data were dereplicated using specific metabolomic software (MetaboScape 4) and 2,782 features were extracted, 1,720 of them being also analyzed on MS/MS mode, supporting molecular networking investigations with Metgem 1.3.6. These metabolites were thus attemptedly identified using GNPS databases for de-novo annotation followed by manual curation (n=84). This data provides essential information on the important sexual dimorphism occurring at the molecular level in the different organ and supports further research on physiology and ecotoxicology in common European prawn.


Steps to reproduce

Gills, muscle, hepatopancreas, nervous gland and gonads; 10 mature males and females; metabolite extraction with 75% methanol; chromatographic separation on Acclaim II column on 5-95% water-ACN gradient prior to ESI+ ionization and analysis on data dependent acquisition mode (2 Hz; 50/1500 m/z) on high resolution qTOF. Raw data were extracted with MetaboScape 4 software using a 5,000-count threshold.


Museum National d'Histoire Naturelle