Metabolomic analysis of acerola cherry
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Untargeted metabolomic analysis An ultra performance liquid chromatography (UPLC) system (SCIEX, UK) was used. An ACQUITY UPLC BEH Amide column (2.1 × 100 mm, 1.7 μm; Waters，UK) maintained at 35 ℃ was used. The mobile phase consisted of solvent A (IPA:ACN = 9:1 + 0.1% formic acid) and solvent B (aqueous solution with 25 mM ammonium acetate and 25 mM NH4H2O). The linear gradient elution procedures were set as follows: 0 ~ 0.5 min, 95% solvent A; 0.5 ~ 9.5 min, 95% to 65% solvent A; 9.5 ~ 10.5 min, 65% ~ 40% solvent A; 10.5 ~ 12.0 min, 40% solvent A; 12.0 ~ 12.2 min, 40% ~ 95% solvent A; 12.2 ~ 15.0 min, 95% solvent A. The flow rate was 0.5 mL/min and the volume injected was 4 μL. A high-resolution tandem mass spectrometer SCIEX Triple-TOF-5600 plus (Applied Biosystems) was used to detect metabolites. The sheath gas flow was set at a pressure of 30 psi, ion source gas 1 and ion source gas 2 were both set at 60 psi, and the interface heater temperature was set at 650 ℃. The ion spray voltages were set at 5000 V and - 4500 V for the positive and negative ion modes, respectively. The mass spectrometry data were acquired in information-dependent acquisition (IDA) mode. The TOF mass range was from 60 to 1,200 Da. During the acquisition, the mass accuracy was calibrated every 20 samples. Furthermore, in order to evaluate the system stability of UPLC-MS/MS analysis, a QC sample was analyzed right after every 10 samples.