Investigating the mechanism of action of aggregation-inducing antimicrobial Pept-ins
Raw lipidomics data, the data set is related to figure 3G-3I.
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Five independent colonies of ancestors, vehicle-treated and P2-resistant colonies from the end of 1/3 MIC treatment were isolated from MH agar plates. They were further inoculated into 200 mL MH medium and grown in the absence of antibiotics for 17 h at 37 °C with shaking. For lipidomic analyses, bacterial culture was collected by centrifugation (7 000 rpm, 4 min) and washed twice with Milli-Q water. The pellet was resuspended in Milli-Q water and snap-frozen in liquid nitrogen. Bacterial sample was resuspended in 20 OD600 units/mL. Cells were lysed with zirconia beads using cell disruptor for 10 mins on ice. The lipids were measured by Lipotype Shotgun Lipidomics service (Lipotype) as previously described (Gerl et al., 2012).