Raw data of Western blot for Figure.3h for manuscript CELL-HOST-MICROBE-D-18-00436R1

Published: 18 August 2018| Version 1 | DOI: 10.17632/9ngf9wmj4f.1
Contributor:
Xuping Xie

Description

As shown in Fig. 3h, the C7a/t virus contained two C variants: HA-C (~12.5 kDa) and C7 (~10.5 kDa) proteins with an estimated molecular ratio of 1:30, whereas the WT ZIKV had only full-length C protein (~11.4 kDa). As expected, both C7a/t and WT viruses contained mature M and E proteins. As controls, culture medium from replicon-transfected cells contained only NS1 protein, with no detectable structural proteins (C, M, or E). The data suggest that C7a/t ZIKV contains both HA-C and C7 proteins. Western blot analysis of C7a/t virus. Supernatants from replicon RNA-transfected BHK-HA-C cells (day 4 p.t.), WT RNA-transfected Vero cells (day 4 p.t.), and C7a RNA-transfected BHK-HA-C cells (day 4 p.t.) were analyzed for viral proteins (HA-C, C, M, E, and NS1) using Western blotting.

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Steps to reproduce

1) Samples: Western blot analysis of C7a/t virus. Supernatants from replicon RNA-transfected BHK-HA-C cells (day 4 p.t.), WT RNA-transfected Vero cells (day 4 p.t.), and C7a RNA-transfected BHK-HA-C cells (day 4 p.t.) were analyzed for viral proteins (HA-C, C, M, E, and NS1) using Western blotting. 2) Reagents: Use SuperSignalâ„¢ West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific) to increase detection

Institutions

University of Texas Medical Branch Department of Biochemistry and Molecular Biology

Categories

Western Blot

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