single-cell RNA-seq of multiple myeloma
Preprocessed single-cell RNA-sequencing data of multiple myeloma. Bone marrow mononuclear cells from a patient with multiple myeloma were purified from bone marrow aspirates by standard density gradient centrifugation using Lympholyte-H (Cedarlane Laboratories Ltd.) and were cryopreserved in CELLBANKER 1 (Takara Bio Inc.). Frozen cells from the multiple myeloma patient were resuspended in RPMI-1640 medium with 10% FBS. The cDNA library was constructed from the sample using the Chromium Single Cell 3′ Library & Gel Bead Kit v2 (10x Genomics) according to the manufacturer’s instructions. The cDNA library was sequenced on an Illumina HiSeq 2500 platform. Alignment to human genome (GRCh38), de-duplication, filtering and generation of gene expression matrixes were performed with the Cell Ranger v3.0.2 (10x Genomics) according to the manufacturer’s instructions.