Rhinoceros Serum MicroRNAs
Description
MiRNA sequences and counts in serum of four species of rhinoceros: black (Diceros bicornis), greater one-horned (Rhinoceros unicornis), Sumatran (Dicerorhinus sumatrensis) and white (Ceratotherium simum). Serum samples from black and Sumatran rhinos were from individuals categorized as healthy, unhealthy (afflicted with disease other than iron overload), and afflicted with iron overload disorder. Serum samples from greater one-horned and white rhinos were from healthy individuals.
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Steps to reproduce
Serum samples (n = 26; 1 -3 samples/individual) from individuals of four rhinoceros species maintained at North American institutions were selected for miRNA sequencing: black rhinos (Diceros bicornis; n = 5 individuals), greater one-horned rhinos (Rhinoceros unicornis; n = 4 individuals), Sumatran rhinos (Dicerorhinus sumatrensis; n = 4), and white rhinos (Ceratotherium simum; n = 4). Samples had been previously banked during veterinary procedures and were used for this study opportunistically. The samples were shipped to LCSciences (Houston, TX) for miRNA sequencing and bioinformatics analysis. Sequences will be submitted for inclusion in the miRBase database, at which point miRNA names may change.