Transcriptome Data for dRTEL1 testes with dRTEL1-GFP
Total RNA was extracted using TRIzol reagent following the manufacturer’s instructions. 40 pairs of w1118 or dRTEL1 or dRTEL1; dRTEL1-GFP early L3 (60-72hr) stage testes were dissected in Schneider's medium (Invitrogen) and used as one set of data respectively. For RNA-Seq, each genotype was sequenced with two replicates. The integrity of RNA was confirmed by gel electrophoresis. Subsequent mRNA purification, library construction, sequencing and data analysis were performed by BGI (Beijing Genomics Institute, Shenzhen, Guangdong, China). In brief, TruSeq RNA V2/Illumina kit was used to generate the Illumina cDNA libraries. Libraries were sequenced with Illumina HiSeq 2500. Raw sequencing reads were cleaned by removing adaptor sequences, reads containing polyN sequences, and low-quality reads. The Drosophila genome (dm6, FlyBase 6.05) was used to align and filter reads.