In search of microbial indicators of marine ecosystem health: using taxonomy as a potential endpoint

Description

Numerical data showing concentrations of metals (As, Ba, Bi, Cd, Co, Cu, Cr, Mn, Ni, Pb, Sb, Sn, Zn - shown as EF= enrichment factors), Hg, TBT, total nitrogen and phosphorus and toxicity level (EC50, Microtox test) measured in 67 surface sediment samples collected in ten polluted areas in the northern Adriatic (Croatia): port of Pula (mark PU), port of Rijeka (mark RI), Raša Bay (mark RA), Bakar Bay (mark BA), and in the southern Adriatic (Croatia): Šibenik Bay (mark SI), eastern part of Kaštela Bay - Vranjic Basin (mark VR), and port of Split (mark ST) as well as in reference areas: Cape Kamenjak (mark CK), Zlarin Island (mark CZ) and Vis Island (mark CV).

Steps to reproduce

Sampling of the surface sediments (0-5 cm, 200-500 mg of sediment) was conducted using a box corer or a Van Veen grab. Sampling was carried out at depths between 2.2 m and 40 m, with a mean sample depth of 14.6 m (± 9.1). The average temperature of the bottom seawater layer was 15.2 °C (± 2.7). Multielement metal analyzes were performed by high-resolution inductively coupled plasma mass spectrometry (HR ICP-MS; Element 2, Thermo, Germany), TBT on the gas chromatograph (GC, Varian CP3800) with pulsed flame photometric detector (PFPD, Varian), TN on a CHNS analyzer (Elementar, Germany) using ISO method 13878:1998, phosphorus using a Shimadzu UV-VIS Spectrophotometer, mercury according to the "in-house" accredited method M 146-200 (edition 1, 15.11.2019) (Adapted method according to the producer manual: AMA 254 Advanced Mercury analyzer Operation manual, 2002) on an instrument AMA254 Mercury Analyzer and the potential toxicity of sediment organic extracts by Microtox© bioassay .

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