ELISA-based quantitations of CCL2 protein levels in the plasma of PGC1(CBM5mut) mice
Description
ELISA-based quantitations of CCL2 protein levels in the plasma of adolescent (1 month), adult (4-6 months), or aged (24 months) PGC1(CBM)5mut/5mut or PGC1(CBM)wt/wt littermates deriving from heterozygous intercrosses. PGC1(CBM)5mut/5mut mice were described in Rambout X, et al. Mol Cell. 2023. Briefly, to evaluate PGC1 function in the context of a living organism, we utilized CRISPR-Cas9 to edit into the mouse genome the five amino acid changes to the PGC1 CBM that preclude CBP80 binding.
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Steps to reproduce
Blood was collected through submandibular bleeding and plasma was separated by centrifugation in EDTA-coated tubes (Greiner Bio-one, Cat# 450476). Absolute plasma CCL2 levels were measured by ELISA using the Mouse CCL2/JE/MCP-1 ELISA Kit (R&D Systems, Cat# MJE00B) following manufacturer’s protocol.