An unconventional integrin 3 and CD103 heterodimer improves the anti-tumor immunity of tissue-resident T cells. Abd Hamid et al.

Published: 13 December 2021| Version 1 | DOI: 10.17632/b2xdk4h5xm.1
Megat Abd Hamid,
Suet Ling Felce,
Chen Jin,
Nasullah Khalid-Alham,
Svenja Hester,
Nikolaos Kanellakis,
David Maldonado-Perez,
Renuka Teague,
Lucia Cerundolo,
Xuan Yao,
Jili Chen,
Benedikt Kessler,
Ricardo Fernandes,
Clare Verrill,
Michael Dustin,
Roman Fischer,
Yanchun Peng,
Tao Dong


Paired CD103+ and CD103- SSX2-specific CD8 T cells and paired CD103+ and CD103- NYESO1-specific CD8 T cells were activated for 0, 3 or 6 hours with anti-CD3/CD28 stimulation. Cells were then lysed in 1% NP40 cell lysis buffer before proteins pelleted and snap freezes. Dried proteins were digested into peptide before analysed by liquid chromatography tandem mass spectrometry (LC-MS/MS) using Ultimate 3000 UHPLC (ThermoFisher Scientific) connected to an Orbitrap Fusion Lumos Tribrid (ThermoFisher Scientific).



University of Oxford


Immunology, Cancer