The First Complete Mitochondrial Genome Data of Hippocampus kuda Originating from Malaysia

Published: 30 March 2020| Version 1 | DOI: 10.17632/b3yjvcn7k2.1
PUTERI NUR SYAHZANANI JAHARI, Nur Fatihah Abdul Malik, Mohd Shahir Shamsir Omar, M. Thomas P. Gilbert, Faezah Mohd Salleh


Mitogenome sequences assembled using raw data for two Malaysian Hippocampus kuda (HK1 and HK2). Genomic DNA was extracted using Qiagen Blood and Tissue Kit (Qiagen, Valencia, CA) following the manufacturer’s instructions. All libraries were prepared using BEST protocol as described in Mak et al., (2017) and were sequenced on an BGISEQ-500 platform (100PE). Raw sequence reads were assembled into individual mitogenome sequence using the MITOBIM v1.8 (reference genome assembly) pipelines. Mitogenome mapping quality was assessed using PALEOMIX and further annotated using MitoAnnotator and GB2sequin annotation web application. The mitogenome data is available in Genbank with the accession numbers MT221436 and MT221436.