Raw data supporting “Data set of the antioxidant properties of ascorbyl 2,6-dipalmitate stabilised nanoemulsions and their facilitated mucodiffusion due to the intestinal-protein corona”

Published: 8 November 2019| Version 1 | DOI: 10.17632/b5y2rm8dv4.1
Contributors:
María Plaza Oliver,
Manuel Santander,
Victoria Lozano Lopez,
L. Castro-Vázquez,
Virginia Rodriguez Robledo,
Joaquin Gonzalez-Fuentes,
Pilar Marcos,
Mariamar Arroyo Jimenez

Description

Colloidal carriers have greatly contributed to overcome biopharmaceutical limitations of drugs with low oral bioavailability, a fact that is related to their physicochemical properties and composition that determine their biological behaviour. In this sense, we have developed α-tocopherol nanoemulsions stabilised by ascorbyl 2,6-dipalmitate (ADP), a hydrophobic derivative of the ascorbic acid, obtaining the formulations NEE1-ADP, NEE2-ADP and NEE3-ADP. In this repository we collect data supporting the document “Data set of the antioxidant properties of ascorbyl 2,6-dipalmitate stabilised nanoemulsions and their facilitated mucodiffusion due to the intestinal-protein corona”, submitted to Data in brief (November 2019). That data set includes results of the antioxidant properties of NEE-ADP formulations, their stability under saline conditions and the mucodiffusion of uncoated and intestinal-protein coated NEE-ADP nanosystems. The raw data of those experiments are included in this repository record. i) Raw data related to ABTS and DPPH assays (please see folder “raw data ABTS and DPPH assays”). Concretely, we have collected the absorbance values measured at 734 nm (ABTS assay) or at 515 nm (DPPH assay). This was the basis of results further detailed in the mentioned dataset (figures 2 and 3). ii) Raw data related to the ccc determination, showing results of the hydrodynamic mean size measurements carried out at increasing concentrations of CaCl2. These data, which can be found in the folder “raw data ccc determination”, were further analysed to obtain figure 4 included in the mentioned data set. iii) Raw data from particle tracking experiments (figure 5 in the related data set). In these studies, raw data consists on time-lapse videos. Samples analysis required 20 videos but we include a selection of 2 videos per sample due to the high size of the files. Please see folder “raw data Particle Tracking experiments” for further details. These videos show the interaction of NEE1-ADP and NEE3-ADP with intestinal porcine mucus, used as model of the human intestinal mucus (sub-folders “Videos NEE1-ADP (nanoemulsion)” and “Videos NEE3-ADP (nanoemulsion)”. Furthermore, we evaluated the effect of the intestinal protein corona in this process (sub-folders “Videos NEE1-ADP (protein-coated)” and “Videos NEE3-ADP (protein-coated)”).

Files

Steps to reproduce

A detailed description of the experimental procedure followed to obtain the raw data included in this repository can be found in the related dataset “Data set of the antioxidant properties of ascorbyl 2,6-dipalmitate stabilised nanoemulsions and their facilitated mucodiffusion due to the intestinal-protein corona”, submitted to Data in brief.