A 3-D Printed Vitrification Device Integrated with French Straws

Published: 5 September 2022| Version 2 | DOI: 10.17632/b7yy72wcxx.2


This data set includes ‘stl’ files that can be used for 3D printing, and ‘ipt’ files that can be used for design modifications in Autodesk Inventor or Fusion 360. More information of this device can be found in the article ‘A 3-D Printed Vitrification Device Integrated with French Straws’ published in the journal Hardware X. (1) Vitrification wands: Vitrification quality is inversely related to sample volume, because higher thermal mass leads to lower cooling rates. Thus, vitrification wands were designed with multiple configurations that differed by loop length and height (different number of layers in 3-D printing slicing). The nominal height per layer was 0.2 mm when printed with 0.4-mm printer nozzles. Loop configurations included three lengths (Short:15.4 mm, intermediate:16.6 mm, and long:24.4 mm) and three heights (1 layers, 2 layers, 3 layers). The total length of the wands was identical to French straws to fit within standard cryogenic goblet storage containers. Wands were printed with polylactic acid (PLA) thermoplastic filament, widely used for 3D printing, and can withstand liquid nitrogen temperatures. (2) Jig apparatus for creation of protective sleeves: A self-contained jig apparatus was designed to size, indent, and cut a standard 0.5-mL cryopreservation straw (IMV Technologies, L'Aigle, France) into two protective sleeves. This apparatus consisted of a dock, a stylus, and a stencil. The stylus featured a tapered tip to produce a defined indentation on French straws that would slide along a groove fabricated in the vitrification wands. The stencil ensured correct positioning of the French straws within the jig and facilitated localization of the indentation. The stylus enabled positioning of a razor blade, stylus, and stencil during sleeve making. The stylus and stencil were self-contained by insertion into the dock after use.


Steps to reproduce

1) Print vitrification wands with desired loop configurations (with recommended settings in the table). 2) Print components of jig apparatus (with recommended settings in the table). 3) Label 0.5-mL French straws at each end (two labeled sleeves will be produced per straw). 4) Sheath a straw onto the stencil. 5) Insert loaded stencil into the dock through the upper opening on the end of the dock. 6) Push the loaded stencil into the dock as far as it will go. 7) Remove the stylus from the dock. 8) Align the stylus in the receiving slot and press to form an indentation on the straw with the tapered tip. 9) Place a single-edge razor blade into the perpendicular slot in the dock and slice the straw into two sections. 10) Repeat the steps above for the other half of the straw to create two protective sleeves. 11) The protective sleeve should be placed on the wand by aligning the indentation with the groove. 12) The sleeve should be in the ‘open’ position (closest to handle) prior to sample loading. 13) The protective sleeve can be slid along the groove into the ‘closed’ position to cover the loops.


Louisiana State University


Vitrification, Storage of Biological Residues