Mitoplate S-1 analysis using R

Published: 15 December 2020| Version 3 | DOI: 10.17632/b9mprfdvmv.3
Contributors:
Flavia Radogna,
Déborah Gérard,
Mario Dicato,
Marc Diederich

Description

This R script performs normalisation of data obtained with the MitoPlate S-1 commercialised by Biolog. In addition, it creates a scatterplot of initial rate values between conditions of interest. The script includes a first normalisation step using the "No substrate" well (A1) required for the rows A to H and a second normalisation step using the "L-Malic Acid 100 µM" (G1) only required for the rows G and H. Initial rate values are calculated as the slope of a linear regression fitted between 30 minutes and 2 hours.

Files

Steps to reproduce

Raw data must be saved as text files (.txt) with the first column being the time, the second column being the temperature and the last columns being the absorbance of the different wells.