Supplementary_files_Comparative_genomic_analysis_chemosensory-related
Description
The starting datasets were represented by files with the nucleotide sequence corresponding to the genome and proteome of three gastropod species, with their respective annotation files. These data are available in the server of the National Center for Biotechnology Information (NCBI); it was not data generated in our work, as we indicated in materials and methods. Then we used the BITACORA v.1.2.1 software to identify genes from chemosensory families, the output of the analysis yielded files with protein sequences identified by this tool. Alignments were made on these sequences with the Mafft v.7.453 software and phylogenetic trees were built with IQTree v.2.1.2. In addition, we run homemade scripts for the identification of gene clusters by measuring the physical distance among genes. Finally, the genetic distances among genes were estimated with the MEGA-CC v.11.0.11 program.