Population structure of green frogs in Western Ukraine according to the results of analysis in Structure, BAPS and NewHybrids
Population structure of green frogs in Western Ukraine according to the results of analysis in Structure, BAPS and NewHybrids. Different colors of bars represent the clusters found by the program. Numbers 3, 32, and 56 are absent on the diagram because DNA was impossible to extract from those samples.
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The analysis of population structure with the implementation of the Bayesian approach was performed in programs Structure 2.3.4, BAPS 6.0, and NewHybrids 1.1. For the clusterization in Structure the K value was set in the range between 2 and 7. Also, 10 runs with the length of burning period equal to 10 000 and 100 000 iterations (МСМС) were set. The optimal number of groups (K) was calculated in Structure Harvester. Multiple results of clusterization from Structure were united in Clumpp to obtain a consensus result. While running BAPS 6.0, according to the recommendations of developers, K value was set equal to 3, 5, 10, and 15. Used settings: the number of iterations to estimate admixture coefficient – 100; the number of referent populations – 200. As developers recommend to select a number of iterations for admixture coefficient estimation in the range between 5 and 20, we used 10. By using the NewHybrids 1.1 program we performed an estimation of the hybridity of individuals in a population based on loci RlCA1b5 and Rrid059A. Those loci were selected because each of them has unique alleles that are fixed in P. lessonae (frequency 1,00). Calculations were done using the following settings: the length of burning period equal to 20 000 and 200 000 iterations (МСМС).