Chronic cysteine, glutamine and glutamic acid supplementation reduces anxiety and depressive-related behaviors in Wistar rats subjected to chronic unpredictable mild stress

Published: 14 June 2024| Version 1 | DOI: 10.17632/bchz7b95cd.1
Daniel Salinas Velarde


This study aimed to evaluate the antidepressant and anxiolytic effect of a nutritional supplement containing glycine, cysteine, and glutamic acid, the precursor amino acids of GSH in rats exposed to a CMS procedure. 32 eight-week-old male Wistar rats were randomly assigned to a control group (CON) and three groups exposed to six weeks of CMS (n = 8 per group), treated with daily i.o. administration of 1) fluoxetine (1 mg/kg), 2) the nutritional supplement (0.8 mg/kg), and 3) water as a vehicle. The sucrose preference test was performed once a week to detect anhedonia (a core symptom of depression); the elevated plus maze (EPM) and locomotor activity tests were performed to detect anxiety-related behaviors. Animals in the CMS groups showed a decrease in weight compared to the CON group. Chronic administration of fluoxetine and the supplement in CMS rats increased sucrose preference and number of entries into open arms, and reduced anxiety index to levels such as the CON group, compared with vehicle-treated CMS rats.


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Universidad de Guadalajara Centro Universitario de Los Lagos


Depression, Anxiety, Behavior (Neuroscience), Behavioral Nutrition