Raw data from: Biocompatible nanocomposite hydroxyapatite-based granules with increased specific surface area and bioresorbability for bone regenerative medicine applications

Published: 25 March 2024| Version 1 | DOI: 10.17632/bcy87d4dsb.1
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Description

Hydroxyapatite (HA) granules are frequently used in orthopedics and maxillofacial surgeries to fill bone defects and stimulate the regeneration process. Optimal HA granules should have high biocompatibility, high microporosity and/or mesoporosity, and high specific surface area (SSA), which are essential for their bioabsorbability, high bioactivity (ability to form apatite layer on their surfaces) and good osseointegration with the host tissue. Commercially available HA granules that are sintered at high temperatures (≥ 900 °C) are biocompatible but show low porosity and SSA (2-5 m2/g), reduced bioactivity, poor solubility and thereby, low bioabsorbability. HA granules of high microporosity and SSA can be produced by applying low sintering temperatures (below 900 °C). Nevertheless, although HA sintered at low temperatures shows significantly higher SSA (10-60 m2/g) and improved bioabsorbability, it also exhibits high ion reactivity and cytotoxicity under in vitro conditions. The latter is due to the presence of reaction by-products. Thus, the aim of this study was to fabricate novel biomaterials in the form of granules, composed of hydroxyapatite nanopowder sintered at a high temperature (1100 °C) and a biopolymer matrix: chitosan/agarose or chitosan/β-1,3-glucan (curdlan). It was hypothesized that appropriately selected ingredients would ensure high biocompatibility and microstructural properties comparable to HA sintered at low temperatures. Synthesized granules were subjected to the evaluation of their biological, microstructural, physicochemical, and mechanical properties. Folder: “Physicochemical properties” contains raw data of the FTIR and thermogravimetric analysis results. A Microsoft Word file (Microhardness test.docx) contains the results of the microhardness test. The results are expressed as Young’s modulus values (GPa) presented in tables for each sample. A Microsoft Excel file (MIP - Porosity.csv) contains the results of porosity evaluation for each tested granule. Folder: “SSA” contains Microsoft Excel files with results of the total surface area measurement performed for each sample. Folder: “Stereoscopic images” contains raw stereoscopic images of the granules. Folder: “SEM” contains raw SEM images of tested granules. Folder: “Ca_P” contains Microsoft Word files with calculated Ca/P atomic ratio for each sample. A Microsoft Excel file (Biodegradation.csv) contains the results of samples weight loss for each tested granule. Folder: “Bioresorption” contains raw CLSM images showing uptake of released from biomaterial nanoHA. A Microsoft Excel file (Absorption.csv) contains the results of samples liquid absorption capacity. Folder: “MTT” contains the raw results of three repetitions of MTT cytotoxicity assay performed after 24 hours of culture. The results of MTT test are expressed as OD values. Folder: “Live_Dead” contains raw CLSM images of the cells on the granules after live/dead staining.

Files

Institutions

Politechnika Lubelska, Uniwersytet Slaski w Katowicach, Akademia Gorniczo-Hutnicza imienia Stanislawa Staszica w Krakowie, Uniwersytet Medyczny w Lublinie

Categories

Biomaterial, Regenerative Medicine, Polymer Nanocomposites, Bone Regeneration

Funding

Narodowe Centrum Nauki

UMO-2021/41/N/NZ7/01633

Licence