Nanopore sequencing data and structural variants identified in Prunus avium seedlings derived through mutagenesis

Published: 6 June 2022| Version 1 | DOI: 10.17632/bd5xhv99n8.1
Contributors:
Per McCord,
,

Description

DNA from sweet cherry seedlings derived from gamma-irradiated mother trees was sequenced via nanopore sequencing, in an effort to identify structural variants that may have arisen from the gamma irradiation. The reads were aligned with the sweet cherry reference genome sequence (v1.0.a1), and both short-medium indels and larger structural variants were identified. This dataset includes the FASTA files containing full-length sequences for the identified indels and structural variants. The header of each FASTA sequence also includes the type of variant.

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Steps to reproduce

Genomic DNA was extracted using a CTAB-based buffer. Fragments <25Kb were removed using a Circulomics Short Read Eliminator Kit. DNA was then sequenced using an Oxford Nanopore Technologies MinION flow cell (two barcoded samples per flow cell, for a total of four samples). Sequence reads were analyzed for variants using CLC Workbench "Indels and Structural Variants" tool.

Institutions

Washington State University

Categories

Mutagenesis, Structural Genomics, Cherry, Genome Sequencing

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