raw images related to Figure 5 of article titled "Live-cell single RNA imaging reveals bursts of translational frameshifting"

Published: 12-06-2019| Version 1 | DOI: 10.17632/bd9vccp26k.1
Kenneth Lyon


u-2 os cells transfected with +FSS 2xMF tag was tracked for ~40 minutes total. Used to k_off kinetics of frameshifting sites. 561 nm (0 frame) and 488 nm (-1 frame) lasers imaged every 5th frame. 647 nm (RNA) lasers used to image every frame. Maximum projection of ~13z slices