raw images related to Figure 5 of article titled "Live-cell single RNA imaging reveals bursts of translational frameshifting"
Published: 12 June 2019| Version 1 | DOI: 10.17632/bd9vccp26k.1
Contributor:
Kenneth LyonDescription
u-2 os cells transfected with +FSS 2xMF tag was tracked for ~40 minutes total. Used to k_off kinetics of frameshifting sites. 561 nm (0 frame) and 488 nm (-1 frame) lasers imaged every 5th frame. 647 nm (RNA) lasers used to image every frame. Maximum projection of ~13z slices
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Fluorescence Microscopy