Data and statistical analysis for research article on Deficiency in MT5-MMP Supports Branching of Human iPSCs-Derived Neurons and Reduces Expression of GLAST/S100 in iPSCs-Derived Astrocytes published in Cells by Arnst et al. (2021)

Published: 6 July 2021| Version 1 | DOI: 10.17632/bfj8pnxfh8.1


The data set includes original gel corresponding to Figure 1B and all measurements and statistical analyses used for other figures in the aforementioned article by Nikita Arnst, Pedro Belio-Maira, Laura García-González, Laurie Arnaud, Louise Greetham, Emmanuel Nivet, Santiago Rivera and Alexander Dityatev. We found that MT5-MMP-deficient neurons exhibited an increased number of primary and secondary neurites, as compared to isogenic hiPSC-derived neurons. Moreover, MT5-MMP-deficient astrocytes displayed higher surface area and volume compared to control astrocytes. The MT5-MMP-deficient astrocytes also exhibited decreased GLAST and S100β expression. These findings provide novel insights into the physiological role of MT5-MMP in human neurons and astrocytes, suggesting that therapeutic strategies targeting MT5-MMP should be controlled for potential side effects on astrocytic physiology and neuronal morphology. GraphPad Prism version 9.0 for Windows (GraphPad Software, San Diego, CA, USA) is necessary to read the data for figures 3, 5 ,6.


Steps to reproduce

As decribed in the aforementioned article.


Deutsches Zentrum fur Neurodegenerative Erkrankungen


Cellular Neuroscience