Plos One Makhija et al 2024
Description
These are the raw images of MSCs from which the data for following figures was generated: Fig. 5B, Fig. S5C,D, Fig. S7, Fig. S8 For each of the 5 donors (LzMSC3, LzMSC5, LzMSC6, LzMSC7, and SCT1), corresponding to each time point (day 3, 6, 9, 12), there are 3 replicate wells included in this data set. Each image comprises of two channels, corresponding to actin stain and DAPI stain for nucleus. Whole-well overview images of actin and nucleus were captured on Olympus IX83 fluorescence microscope using 4X objective and tile-scan option in Metamorph software. Individual tiles were 2048 x 2048 pixels, pixel size was 1.6 microns. Image tiles were stitched using the “Stitching” plugin in ImageJ. Individual wells were then cropped manually from the stitched images using the circular cropping tool in ImageJ (radius 3636 pixels). Note that the stitching often generated artefact in the form of high intensity at the junctions between neighboring tiles. However, we observed that these intensity artefacts did not affect quantification of coherency from whole-well actin images.