Morao_et_al_2022
Description
Imaging data in "Topoisomerases I and II facilitate condensin DC translocation to organize and repress X chromosomes in C. elegans" Ana Karina Morao1, Jun Kim1, Daniel Obaji1, Siyu Sun1 and Sevinc Ercan1 1 Department of Biology, Center for Genomics and Systems Biology, New York University, New York, NY 10003, USA
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Steps to reproduce
top-1::degron::GFP and top-2::degron::GFP worms incubated with and without auxin were aligned side by side prior to imaging. For this, 5 µL of 100 mM sodium azide were added to a 2% agar pad positioned on top of a coverslip and 4-6 L2/L3 larvae were transferred to the liquid and aligned using a hair pick (Figure 3B). For glp-1 mutants (Figure S1B), adult worms grown at the permissive (16°C) and restrictive (25°C) temperatures were immobilized with 100 mM sodium azide immediately before imaging. Images were acquired with a Zeiss Axio Imager A2 microscope using a 10x and 20x objective and the AxioVision Rel.4.8 software.