Research Data for "Two genes, ANS and UFGT2, from Vaccinium spp. are key steps for modulating anthocyanin production ".

Published: 20 January 2023| Version 1 | DOI: 10.17632/bkyvdgfj3c.1
Catrin Guenther,


Anthocyanins are a major group of red to blue spectrum plant pigments with many consumer health benefits. Anthocyanins are derived from the flavonoid pathway and diversified by glycosylation and methylation, involving the concerted action of specific enzymes. Blueberry and bilberry (Vaccinium spp.) are regarded as ‘superfruits’ owing to their high content of flavonoids, especially anthocyanins. While ripening-related anthocyanin production in bilberry (V. myrtillus) and blueberry (V. corymbosum) is regulated by the transcriptional activator MYBA1, the role of specific structural genes in determining the concentration and composition of anthocyanins has not been functionally elucidated. We isolated three candidate genes, CHALCONE SYNTHASE (VmCHS1), ANTHOCYANIDIN SYNTHASE (VmANS) and UDP-GLUCOSE : FLAVONOID-3-O-GLYCOSYLTRANSFERASE (VcUFGT2), from Vaccinium, which were predominantly expressed in pigmented fruit skin tissue and showed high homology between bilberry and blueberry. Agrobacterium-mediated transient expression of Nicotiana benthamiana showed that overexpression of VcMYBA1 in combination with VmANS significantly increased anthocyanin concentration (3-fold). Overexpression of VmCHS1 showed no effect above that induced by VcMYBA1, while VcUFGT2 modulated anthocyanin composition to produce delphinidin-3- galactosylrhamnoside, not naturally produced in tobacco. In strawberry (Fragaria × ananassa), combined transient overexpression of VcUFGT2 with a FLAVONOID 3´,5´- HYDROXYLASE from kiwifruit (Actinidia melanandra) modulated the anthocyanin profile to include galactosides and arabinosides of delphinidin and cyanidin, major anthocyanins in blueberry and bilberry. These findings provide insight into the role of the final steps of biosynthesis in modulating anthocyanin production in Vaccinium and may contribute to the targeted breeding of new cultivars with improved nutritional properties. These raw datasets underly gene expression (RT-PCR) analyses and functional characterization (anthocyanin concentrations) of gene candidates using heterologous expression in plantae as published in Front. Plant Sci. 14:1082246. doi: 10.3389/fpls.2023.1082246


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All methods underlying the generation of these data are outlined in the following article: Nguyen HM, Putterill J, Dare AP, Plunkett BJ, Cooney J, Peng Y, Souleyre EJF, Albert NW, Espley RV and Günther CS (2023) Two genes, ANS and UFGT2, from Vaccinium spp., are key steps in modulating anthocyanin production. Front. Plant Sci. 14:1082246. doi: 10.3389/fpls.2023.1082246


New Zealand Institute for Plant and Food Research Ltd, University of Auckland


Molecular Biology, Gene Expression, Secondary Metabolite


Ministry for the Environment, New Zealand

contract C11X1704 “Filling the Void: boosting the nutritional content of New Zealand fruit.”