Maternal β-hydroxybutyrate during the dry period is associated with altered epithelial cell regulation and rumen morphology of offspring as calves-Supplemental Tables

Description

This dataset contains supplementary tables supporting the manuscript entitled “Maternal β-hydroxybutyrate during the dry period is associated with altered epithelial cell regulation and rumen morphology of offspring as calves.” The file includes: (1) ingredient and chemical composition of the total mixed ration fed to dry cows; (2) primer sequences and qPCR conditions for genes related to rumen epithelial integrity, metabolic regulation, inflammation, and lipid metabolism; (3) maternal growth performance and serum β-hydroxybutyrate concentrations during the dry period; and (4) rumen morphological and growth-related traits of newborn and one-month-old calves. These data provide detailed methodological and quantitative information supporting the results reported in the published article.

Steps to reproduce

Cows were monitored during the dry period (−7, −5, −3, and −1 wk before calving) for serum β-hydroxybutyrate (BHB) concentrations. Based on average BHB levels, cows were classified into low-BHB (CON) and high-BHB (MB) groups using the median value as the cutoff. Body weight and body condition score were recorded weekly. Calves were euthanized at birth (24 h) or at one month of age. Rumen tissues were collected from the ventral sac for histological and molecular analyses. For morphological assessment, tissues were fixed in 4% paraformaldehyde, paraffin-embedded, sectioned (4–5 μm), stained with hematoxylin and eosin, and analyzed using light microscopy and ImageJ software to measure papilla length, width, epithelial layer thickness, and rumen mass. Total RNA was extracted from rumen tissue using TRIzol reagent, and cDNA was synthesized for quantitative PCR analysis. Gene expression was quantified using SYBR Green chemistry and normalized to 18S rRNA and GAPDH using the 2^-ΔΔCt method. Primer sequences and amplification conditions are provided in Supplementary Table 2. Maternal growth performance data and rumen morphological measurements were analyzed using linear mixed-effects or fixed-effects models in R. The supplementary tables contain raw diet composition, primer details, maternal metabolic data, and calf rumen morphological measurements used to support the results reported in the manuscript.

Institutions

• Nanjing Agricultural University

  Jiangsu, Nanjing

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