Protein dataset of immortalized keratinocyte HaCaT cells and normal human keratinocytes

Published: 19 January 2021| Version 1 | DOI: 10.17632/bpck4nwdv9.1
Contributors:
Tatiana Butkova,
,
,
,

Description

Learning of the molecular mechanisms of the pathological processes development in the normal human keratinocytes (NHK) are difficult. These cells are aging in culture - their phenotype, and as a consequence, the protein composition, undergoes changes with an increase in the number of passages, and the duration of cultivation itself is limited. Another known disadvantage of using primary cell lines as an object of analysis is the significant variability in the structural and functional characteristics of cells obtained from different donors. Immortalized keratinocytes HaCaT are often used as an analogue of NHK since they have a number of advantages over the latter - they do not require the presence of growth and differentiation factors in the medium, have unlimited potential for proliferation, demonstrate stable phenotype regardless of the number of passages (Colombo et al., 2017). Certainly, due to the fact that HaCaT is an immortalized cell line, some of their properties distinguish them from NHK (Boukamp et al., 1988). However, HaCaT keratinocytes have key properties similar to NHK. For example, HaCaT cells exhibit a similar response to differentiation stimuli - increased Ca2 + concentration (Deyrieux et al., 1988) and high confluence (Capone et al., 2000) cause HaCaT to produce keratinocyte differentiation factors such as the cytokeratins K14, K10; Involucrin (Colombo et al., 2017). These cells can form the stratum corneum of the epidermis under the influence of transforming growth factor-α and interleukin-1 (Szabowski et al., 2003). Taking into account the properties and characteristics of the HaCaT line, these cells can be considered as a promising experimental model for research various physiological processes occurring in human keratinocytes. However, to understand the limitations of such an experimental model, a detailed comparative characterisation of HaCaT and NHK is required, which can be obtained by carrying out its proteomic analysis.

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Institutions

Naucno-issledovatel'skij institut biomedicinskoj himii imeni V N Orehovica

Categories

Liquid Chromatography Mass Spectrometry, Extraction Methods, Data Processing, Cell Cultivation

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