Data for: Effect of He-Ne laser irradiation on embryonic development in chicken eggs Short title: Photobiomodulation and chicken eggs
Description
This study investigated the influence of He-Ne laser irradiation at different doses during the developmental process of chicken embryos. Freshly fertilized chicken eggs were exposed during the first 48 hours post-laying to laser radiation at a wavelength of 632 nm with different laser doses (0.1, 0.2, 0.3, 1 and 2 mJ/cm2) for different times (20-400 secs) from a He-Ne laser. Following exposure, the eggs were incubated till the 11th day without laser radiation, when the. embryos were removed from the eggs and studied blind. Each of the exposed groups revealed some developmental anomalies compared with the respective sham–exposed control group. There was some dose-response evident with the highest incidence of abnormalities being 6/50 or 12% in the 2 mJ/cm2 group. Histopathological features were examined in the chick embryos using optical microscopy of stained slides. The distribution of naturally occurring apoptosis in chick embryos was studied by electron microscopic imaging. Significantly elevated levels of circulating cytokines (IL-2 and INF- γ) were found in exposed groups compared to sham–exposed embryos. Collectively, these results suggest that exposure to the higher doses of laser can provoke irreparable developmental alterations, and may have implications for photobiomodulation therapy.