Data from proteomic diversity in a prevalent human-infective Giardia duodenalis sub-species

Published: 10 July 2018| Version 3 | DOI: 10.17632/brhg8bhcvm.3
Contributors:
Samantha Emery,
Daniel Vuong,
Ernest Lacey,
Staffan Svard,
Brendan Ansell,
Aaron Jex

Description

Giardia duodenalis a species-complex of gastrointestinal protists, with assemblage A and B infective to humans. To date, post-genomic proteomics are largely derived from Assemblage A, biasing understanding of parasite biology. To address this gap, we quantitatively analysed the proteomes of trophozoites from the genome reference and two clinical Assemblage B isolates, revealing lower spectrum-to-peptide matches in non-reference isolates, resulting in significant losses in peptide and protein identifications, and indicating significant intra-assemblage variation. We also explored differential protein expression between in vitro cultured subpopulations putatively enriched for dividing and feeding cells, respectively. This data is an important proteomic baseline for Assemblage B, highlighting proteomic differences between physiological states, and unique differences relative to Assemblage A. The complete raw files and search results can be accessed via the ProteomeXchange Consortium (Vizcaino et al., 2013) via the PRIDE partner repository with the dataset identifier PXD007943.

Files