Supplementary Data S1: Next generation sequencing from Hepatozoon canis (Apicomplexa: Coccidia: Adeleorina): Complete apicoplast genome and multiple mitochondrion-associated sequences

Published: 11 February 2019| Version 1 | DOI: 10.17632/bs2z92449s.1
Contributors:
Alexandre Léveillé,

Description

These files comprise all of the NGS sequence assemblies referred to in the article: "Next generation sequencing from Hepatozoon canis (Apicomplexa: Coccidia: Adeleorina): Complete apicoplast genome and multiple mitochondrion-associated sequences." All assemblies were generated from Illumina HiSeq 2500 sequencing data (126 bp paired-end reads, insert length ~500 bp). In the case of mitochondrion-associated sequences 1, 2, 3 and 4: PCR and Sanger sequencing data were utilized to provide additional assembly coverage of CDS regions. Files included are: BAM assembly files: .bam, .bai and .fasta (these files are needed together to generate a BAM assembly flat file - supported by many software platforms). Geneious assembly files: Complete annotated assemblies (with NGS read pairings) can be viewed with Geneious software (versions 6.1 or newer). These files will provide the greatest details of the assembly data. Jpeg images of Geneious assemblies: These files were provided for ease of viewing and rapid analysis. Note: images were not generated for the complete ribosomal DNA unit and 18S rDNA variant assemblies as these assemblies were too large to viewed as images.

Files

Steps to reproduce

Please see the associated journal article.

Institutions

University of Guelph Ontario Veterinary College, University of Guelph Ontario Department of Pathobiology

Categories

Molecular Biology, Bioinformatics, Canine Parasitology, Apicomplexa, Next Generation Sequencing, Genome Sequencing

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