High-Performance Liquid Chromatography Method Modification for the Simultaneous Analysis of Disodium Guanylate and Disodium Inosinate in Mushrooms (specifically Hericium erinaceus)
Description
The data presents a modified method from existing methods for the simultaneous analysis of disodium guanylate (GMP) and disodium inosinate (IMP) in Hericium erinaceus mushrooms using high-performance liquid chromatography (HPLC).
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Mobile phase composition = 10 mM KH2PO4 buffer : MeOH (90 % : 10 %, pH ≈ 4.60 ± 0.05) Column = C18, 4.6 × 250 mm Flow rate = 0.8 mL/min Injection volume = 10 µL Detection wavelength = 254 nm Column temperature = 30 °C Gradient program = Isocratic Note: for standard solution preparation and depending on the concentration to be prepared, appropriate volumes of the selected mobile phase solution and 0.1 M HCl solution should be used to mix GMP and IMP standards, respectively.
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Funding
Ministry of Higher Education
FRGS/1/2023/WAB04/UMS/02/7
Universiti of Malaysia Sabah
GUG0636-2/2023