Fluorescent measurements of the endotoxin adsorption efficiency

Published: 31-08-2019| Version 2 | DOI: 10.17632/by89r75vs7.2
Ekaterina Kholina,
Alexey Nesterenko


We present the technique for the measurement the LPS statical adsorption on the particles in the area of relatively low concentrations. Direct photon count allows us to detect LPS concentration securely from 100 pM. Using this technique we demonstrate how preequilibration pH affects the results. Raw adsorption data sufficient for building adsorption isotherm is also presented for two sorbents.