SUMOylated DDK Prevents Fork Uncoupling and Promotes Rad51-Mediated Replication of Damaged Templates

Published: 14 April 2022| Version 1 | DOI: 10.17632/c284kmxv6x.1


DNA damage tolerance (DDT), activated by replication stress during genome replication, is mediated by translesion synthesis and homologous recombination (HR). Here we uncover that DDK kinase, essential for replication initiation, is critical for replication-associated recombination-mediated DDT. DDK relies on its multi-monoSUMOylation to facilitate HR-mediated DDT and optimal retention of Rad51 recombinase at replication damage sites. Impairment of DDK kinase activity, reduced monoSUMOylation and mutations in the putative SUMO Interacting Motifs (SIMs) of Rad51 impair replication-associated recombination and cause fork uncoupling with accumulation of large single-stranded DNA regions at fork branching points. Notably, genetic activation of salvage recombination rescues the uncoupled fork phenotype but not the recombination-dependent gap-filling defect of DDK mutants, revealing that the salvage recombination pathway operates preferentially proximal to fork junctions at stalled forks. Overall, we uncover that SUMOylated DDK acts with Rad51 and its SIMs in an axis that prevents replication fork uncoupling and mediates recombination-dependent gap-filling.



Fondazione Istituto FIRC di Oncologia Molecolare


Genetics, Yeast, Western Blot, Real-Time Polymerase Chain Reaction, Gel