Transcriptomic analysis of a mouse model of intrahepatic cholestasis

Published: 13 September 2019| Version 1 | DOI: 10.17632/c2zvxm27yc.1
Adriana Carino,


The Farnesoid X receptor (FXR), is the main bile acids sensor expressed in hepatocytes. Obeticholic acid (OCA), a fairly potent FXR agonist, has been approved for the treatment of UDCA-resistant PBC, but FXR gene ablation protects against liver injury in cholestatic modes. The use of OCA causes itching in a substantial proportion of PBC patients, and at the dose 5 mg/d, has been linked to a cluster of severe liver injury, in some cases fatal, in PBC patients with decompensated cirrhosis and Child-Pugh Class B/C. To further elucidate the role of FXR in the pathogenesis of cholestasis, we have tested the obeticholic acid alone or in combination with a FXR antagonist, named as GP7, in a mouse model of ANIT (α-naphthylisothiocyanate) induced cholestasis. OCA and GP7 intrahepatic effect were evaluated by RNAseq analysis performed on Ion S5 Sequencer with Torrent Suite Software v6.



Universita degli Studi di Perugia


Gastroenterology, RNA Sequencing, Cholestasis