Study on the efficient utilization mechanism of dietary starch by swamp eel (Monopterus albus)

Published: 16 May 2022| Version 1 | DOI: 10.17632/c34snkx5ky.1
Contributor:
Chuanqi Yu

Description

Swamp eel (Monopterus albus), a commercially farmed and carnivorous freshwater fish, possess high efficiency on the utilization of dietary starch, but the underlying mechanism remain elusive. Therefore, we aimed to explore how does swamp eel efficiently utilize dietary starch. Firstly, we obtained the transcriptome of the fish by next generation sequencing (NGS) and de novo assbemby. We annotated the unigene of the transcriptome and analyzed differentially expressed genes (DEG) in intestine of fish fed with two levels of starch (20%-NF and 32%-HF) diets. Secondly, we tested the microbial community of the digesta included in intestine of swamp eel fed HF and NF diets through 16S rDNA gene high throughput sequencing analysis. Finally, we calculated the concentrates of short-chain fatty acids (SCFAs) of the digesta included in intestine of swamp eel fed HF and NF diets by GC/MS analysis. Several important DEG, microbial community and SCFAs were identified to be responsible for the high efficiency on the utilization of dietary starch in swamp eel. File S1: DEG in intestine of swamp eel between treatments; File S2: Microbial community in intestine of swamp eel between treatments; File S3: The concentrates of short-chain fatty acids (SCFAs) in intestine of swamp eel between treatments.

Files

Steps to reproduce

High starch diets, carnivorous fish, intestine, omics

Institutions

Jiangxi Agricultural University

Categories

Natural Sciences

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