Myricetin on Triethylene Glycol Dimethacrylate Induced Matrix-Metalloproteinases Expression in Odontoblast cells Data

Description

The following data presents the results obtained from each of the assays conducted in the study titled: Myricetin Modulate Matrix-Metalloproteinases Expression Induced by TEGDMA in human Odontoblast-like Cells. On the first sheet titled "Chromatography," the data corresponds to the evaluation of the presence of test substances in the culture medium, obtained through liquid cromatography coupled with mass spectrometry (LCMS). The second sheet titled "PCR" contains data related to the assessment of mRNA expression for MMP-2 and MMP-9, obtained through real-time polymerase chain reaction (RT-qPCR) from total RNA extracted from odontoblast-like cells exposed to 4-aminophenylmercuric acetate (APMA), hydrogen peroxide (H2O2), proanthocyanidin (PAC), myricetin (MYR), and triethylene glycol dimethacrylate (TEGDMA). On the third sheet titled "ELISA," you will find data regarding the assessment of MMP-2 and MMP-9 concentrations in supernatants from cultures of odontoblast-like cells exposed to Phorbol 12-myristate 13-acetate (PMA), H2O2, PAC, MYR, and TEGDMA. This information was obtained through an Enzyme-Linked Immunosorbent Assay (ELISA). The fourth sheet titled "MMP Activity Data" contains data pertaining to the assessment of MMP-2 and MMP-9 activity in supernatants from cultures of odontoblast-like cells exposed to ethylenediaminetetraacetic acid (EDTA), APMA, H2O2, PAC, MYR, and TEGDMA. This data was obtained through a Fluorescence Resonance Energy Transfer (FRET) assay. The data support the ability of myricetin to transcriptionally control the expression of MMP-2 and MMP-9 induced by contact with TEGDMA in odontoblast-like cells in a cellular model of dentin-odontoblastic cell coculture.

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