A dataset of clinical parameters of infertile women undergoing IVF, including FSHB c.-211G>T and FSHR c.2039A>G genotypes
A dataset of clinical parameters of infertile women undergoing through ovarian stimulation for assisted reproductive technologies in Rostov-on-Don, Russia. A short gonadotropin-releasing hormone antagonist protocol was used for ovarian stimulation. Early-follicular-phase follicle-stimulating hormone, luteinizing hormone, anti-mullerian hormone and progesterone levels were assayed in serum on 3-5-th menstrual cycle day 5-6 cycles before ovarian stimulation, with “Access hFSH”, “Access hLH”, “Access AMH” and “Access Progesterone” kits using Automatic Immunochemical Analyzer “Access 2” (Beckman Coulter, USA). Genotyping of FSHB c.-211G>T [rs10835638] genetic variant was performed using restriction fragment length polymorphism (RFLP) analysis with restriction endonuclease TatI and primers: 5´- GGAGCCAGATCATGAAATGTT-3´, 5´- GACCAATGCTAGCCTGAAGC-3´. Genotyping of FSHR c.2039A>G [rs6166] genetic variant was performed using RFLP analysis with restriction endonuclease BsrI and primers: 5´-TTTGTGGTCATCTGTGGCTGC-3´, 5´-CAAAGGCAAGGACTGAATTATCATT-3´. The PCR products detection was performed by horizontal electrophoresis in 2% agarose gel. All samples were validated by double genotyping.
Ministry of Science and Higher Education of the Russian Federation