Monitoring Binding and Target-search Dynamics of Epigenetic Regulatory Factors Using Live-Cell Single-Molecule Tracking
Description
Video S1: Example population single-molecule tracking experiment. HT-CBX2 is sparsely labeled in mES cells and tracked via stroboscopic bursts to determine fraction of stably- and transiently-bound molecules. Video S2: Example residence single-molecule tracking experiment. HT-CBX2 is sparsely labeled in mES cells and tracked via long integration times to determine the residence time of bound molecules. Video S3: Example angular distribution single-molecule tracking experiment. HT-CBX2 is labeled in mES cells using TMR to locate condensates. HT-CBX2 is also sparsely labeled by JF646 to track individual molecules to determine both the number of molecules inside and outside of condensates as well as angular distribution of molecules in relation to their condensate occupation status. Image S1 (related to Video S3): Epifluorescence of HT-CBX2 labelled with TMR before single-molecule tracking. Image S2 (related Video S3): Epifluorescence of HT-CBX2 labelled with TMR after single-molecule tracking.
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Steps to reproduce
These data are collected by using live-cell single-molecule tracking.