Data from a performance comparison of four commercially available flow cytometers using polystyrene beads

Published: 11 Jan 2019 | Version 1 | DOI: 10.17632/c7nh26z8p3.1
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Description of this data

Accurate comparison of flow cytometric data requires an understanding of how the cytometric fingerprint of a sample may vary from instrument to instrument. Key sources of variability include the number, wavelengths, and power of excitation lasers; the number and types of emission detectors; sample-handling systems and options; and whether fixed or dynamic detector voltages are used. To explore this variability, identical suspensions of three sizes (0.2, 0.5, and 0.8 m-diameter) of solid, fluorescent, polystyrene beads were prepared. The suspensions were then run at on four commercially available flow cytometers, keeping instrument settings as consistent as possible. The results are displayed graphically in Figure 3 of the article “Flow cytometry applications in water treatment, distribution, and reuse: A review” (DOI: 10.1016/j.watres.2018.12.016). This dataset contains the complete .FCS files generated from the experimental comparison.

Experiment data files

Latest version

  • Version 1

    2019-01-11

    Published: 2019-01-11

    DOI: 10.17632/c7nh26z8p3.1

    Cite this dataset

    Safford, Hannah (2019), “Data from a performance comparison of four commercially available flow cytometers using polystyrene beads”, Mendeley Data, v1 http://dx.doi.org/10.17632/c7nh26z8p3.1

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Institutions

University of California Davis

Categories

Flow Cytometry, Water Monitoring

Licence

CC BY 4.0 Learn more

The files associated with this dataset are licensed under a Creative Commons Attribution 4.0 International licence.

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This dataset is licensed under a Creative Commons Attribution 4.0 International licence. What does this mean? You can share, copy and modify this dataset so long as you give appropriate credit, provide a link to the CC BY license, and indicate if changes were made, but you may not do so in a way that suggests the rights holder has endorsed you or your use of the dataset. Note that further permission may be required for any content within the dataset that is identified as belonging to a third party.

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