Differential proteomic analysis of laser-microdissected penetration glands of avian schistosome cercariae with a focus on proteins involved in host invasion
Description
The data are supplementary to the article "Differential proteomic analysis of laser-microdissected penetration glands of avian schistosome cercariae with a focus on proteins involved in host invasion" published in the International Journal for Parasitology 2022, vol. 52, issue 6, pp 343-358, https://doi.org/10.1016/j.ijpara.2021.12.003. The tables include mainly peptide identifications and annotations based on LC-MS/MS analyses of proteins contained in two separately isolated types of penetration glands of infectious larvae of the avian parasite Trichobilharzia szidati (Trematoda: Schistosomatidae) causing cercarial dermatitis in humans. Results of optimization experiments are also presented. Schistosome invasive stages, cercariae, leave their intermediate snail hosts, penetrate the skin of definitive hosts, and transform to schistosomula migrating to final location. During invasion, cercariae employ histolytic and other bioactive products of specialized holocrine secretory cells – postacetabular and circumacetabular penetration glands, the content of which is unsufficiently characterized. We present here the largest dataset known so far concerning the representation and localization of cercarial penetration gland proteins. We optimized the methods of sample processing with cercarial bodies (heads) first. Alizarin-pre-stained, chemically non-fixed samples provided optimal results of MS analyses, and enabled distinguishing PA and CA glands for laser microdissection. Peptidases of 5 catalytic types accounted for ca. 8 % and 6 % of reliably identified proteins in postacetabular and circumacetabular glands, respectively. Invadolysin, nardilysin, cathepsins B2 and L3, and an elastase 2b ortholog were the major gland endopeptidases. Two cystatins and a serpin were highly abundant peptidase inhibitors in the glands. While postacetabular glands generally had a rich enzymatic equipment, circumacetabular glands were conspicuously abundant in venom allergen-like proteins (PF00188 protein family members).