Bacterial Diversity Shapes Carbon, Nitrogen, and Phosphorus Stoichiometry in Fine Roots of Dominant Plants in Mountain Ecosystems

Published: 17 April 2025| Version 1 | DOI: 10.17632/cbj34kcbbr.1
Contributor:
Yulong Ma

Description

Physicochemical properties of rhizosphere soil, C, N, and P stoichiometric characteristics of fine roots, rhizosphere microbial community and C, N, and P cycling functional gene data of dominant plants at different altitudes in Helan Mountain (for the restoration and sustainable development of mountain ecosystems).

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Soil pH was measured using an electrode pH meter (pHS25, China), and electrical conductivity (EC) was determined using the extraction electrode method (DDS-11A, China) with a water–soil ratio of 2.5:1, and soil moisture (SM) was assessed using the drying method. Available potassium (AK) was analyzed using the CH₃COONH₄ extraction-flame photometer technique. Alkaline hydrolyzable nitrogen (AN) was measured through the alkaline hydrolysis diffusion method. Using the potassium dichromate external heating method, soil organic carbon (SOC) was determined. Total nitrogen (TN) was measured with an automatic Kjeldahl nitrogen analyzer (K-360, BUCHI Labortechnik AG, Switzerland), whereas total phosphorus (TP) was quantified using the HCLO4-H2SO4 method combined with molybdenum antimony resistance colorimetry (UV1600 UV-visible spectrophotometer, China)

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Botany, Soil Microbiology

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