qPCR data for reference gene selection in tomato leaf tissue infected with Tomato curly stunt virus

Published: 23 Jan 2020 | Version 1 | DOI: 10.17632/ccb5mkpkz5.1
Contributor(s):
  • Farhahna Allie,
    Unspecified
    Principal investigator
  • Imanu Mwaba,
    Biochemistry, Genetics and Molecular Biology
    University of Johannesburg
    Data collection, Data analysis
  • Mamokete Bokhale
    Mamokete Bokhale
    University of Johannesburg
    Conducted qPCR experiments, Data acquisition and Analysis

Description of this data

qPCR data for Actin7 (ACT), Β-6 Tubulin (TUB), Ubiquitin 3 (UBI), Clathrin adaptor complexes medium subunit (CAC), Phytoene desaturase (PDS), Expressed protein (EXP), Glyceraldehyde-3-phosphate dehydrogenase(GAPDH), Adenine phosphoribosyl transferase-like protein (APT1), TAP42-interacting protein (TIP41) and Elongation factor 1-alpha (EF1α) , measure in tomato leaf tissue infected with Tomato curly stunt virus.

Experiment data files

Steps to reproduce

Tomato gene sequences for Actin7 (ACT), Β-6 Tubulin (TUB), Ubiquitin 3 (UBI), Clathrin adaptor complexes medium subunit (CAC), Phytoene desaturase (PDS), Expressed protein (EXP), Glyceraldehyde-3-phosphate dehydrogenase(GAPDH), Adenine phosphoribosyl transferase-like protein (APT1), TAP42-interacting protein (TIP41) and Elongation factor 1-alpha (EF1α) were obtained from the Sol Genomics network (https://solgenomics.net/). FASTA sequence for each gene was used for qPCR prime design using the intergrated DNA technologies PrimerQuest tool. RNA was extracted from resistant and Susceptible leaf tissue infected with Tomato curly stunt virus RNA miniprep kit (Zymo Research, USA) as per manufacturers instruction. RNA was reverse transcribed to cDNA using Maxima H Minus First Strand cDNA Synthesis kit RT-qPCR (Thermo scientific, USA). ). Primer efficiencies were obtained for each candidate genes using dilutions of cDNA and a standard curve was generated from five 10-fold dilution series on The CFX connect™ Real-Time System (Biorad, USA). Melt curve analysis was conducted for each candidate gene using the Bio-Rad CFX manager 3.1

Latest version

  • Version 1

    2020-01-23

    Published: 2020-01-23

    DOI: 10.17632/ccb5mkpkz5.1

    Cite this dataset

    Allie, Farhahna; Mwaba, Imanu; Bokhale, Mamokete (2020), “qPCR data for reference gene selection in tomato leaf tissue infected with Tomato curly stunt virus”, Mendeley Data, v1 http://dx.doi.org/10.17632/ccb5mkpkz5.1

Statistics

Views: 41
Downloads: 0

Institutions

University of Johannesburg Department of Biochemistry

Categories

Molecular Biology, Plant-Pathogen Interaction, Plant Diseases

Licence

CC BY 4.0 Learn more

The files associated with this dataset are licensed under a Creative Commons Attribution 4.0 International licence.

What does this mean?
You can share, copy and modify this dataset so long as you give appropriate credit, provide a link to the CC BY license, and indicate if changes were made, but you may not do so in a way that suggests the rights holder has endorsed you or your use of the dataset. Note that further permission may be required for any content within the dataset that is identified as belonging to a third party.

Report