Combinatorial metabolic engineering of Bacillus amyloliquefaciens to produce spermidine by sustainable utilization of xylose and feather meal
Description
Figure2 The effect of enhancing spermidine synthesis pathway. (a) Effects of different pathways on the synthesis of spermidine. (b) Effects of different genes on spermidine synthesis. Figure 3 The Effect of blocking spermidine degradation on the synthesis of spermidine. (a) Effects of different spermidine degradation genes on spermidine synthesis. (b) Effects of different multi-genes combination regulation strategies on spermidine synthesis. Figure 4 The effect of increasing the supply of precursors on the synthesis of spermidine. (a) The effect of exogenous addition of different precursors on the synthesis of spermidine. The CK group represents the fermentation medium without added precursors. (b) The effect of HSPM3 on the synthesis of spermidine. Figure 5 The effect of carbon source on the synthesis of spermidine in HSPM3. (a) The effect of different types of carbon sources on the spermidine synthesis and cell growth. (b) The effect of different concentrations of xylose on the spermidine synthesis and cell growth. (c) Process curve of cell growth with added 1% xylose at the 24th hour of fermentation. (d) Process curve for the synthesis of spermidine with added 1% xylose at the 24th hour of fermentation. Figure 6 The influence of feather meal as a nitrogen source on the production of spermidine. (a) The effect of feather meal with different concentrations on the synthesis of spermidine by strain HSPM3. The CK group indicates that the fermentation medium with 20 g/L of corn meal. (b) The effect of enhanced alkaline protease expression on the production of spermidine in feather meal culture medium. Figure S1 Transcription level changes of speE and speB in PM1 and PM1-P43speEB. Figure S2 Transcription level changes of speD and speA.(a) Transcription level changes of speD in PM1 and PM1-P43speD. (b)Transcription level changes of speD in PM1 and PM1-P43speA. Figure S3 The effect of replacing arginine operon with promoter on the synthesis of spermidine. Figure S4 The effect of different concentrations of methionine on the synthesis of spermidine.