"Two-headed" juvenile of Nematodirus sp. from reindeer (Rangifer tarandus)
Description
Dataset consists of two folders. Each one contains photo and video of "two-headed" juvenile of Nematodirus sp., obrained from the feces of reindeer (Rangifer tarandus). The folder entitled "Egg in tap water" contains photo and video taken with x10 and x40 magnification, bright field, dark field, phase contrast. The folder entitled "Juvenile in tap water (2 month later)" contains photo and video taken with x10, x20, x40 and x100 magnification, bright field, dark field, phase contrast. This dataset is a supplement for the research article "Double trouble: ‟two-headed” Nematodirus sp. juvenile from R. tarandus" by Olga Loginova for the Russian Journal of Nematology.
Files
Steps to reproduce
The “two-headed” juvenile reported herein was found within an egg during coprological survey of helminth infections in reindeer (Rangifer tarandus) across Palearctic started by author back in 2018. A fecal sample containing the egg in question was excreted by semi-domestic reindeer in Chukotka (Russia). The sample of feces was collected in October 2022 at 65°90’61.39” N; -178°84’64.36” E, delivered to Moscow and studied in December 2022 following procedures published in the National Standard of the Russian Federation GOST R 54627-2011 Ruminant animals. Methods of Laboratory Helminthological Diagnostics, namely sedimentation ovoscopy in tap water. Light micrographs and videos of the egg containing “two-headed” juvenile were taken using an optical microscope Micmed-6 (LOMO-MA, Russia) equipped with phase contrast and dark field optics FATEK M 6-7 (LOMO-MA, Russia) under bright field, dark field, and phase contrast illumination using objective lenses with 10× and 40×. Images and videos were made using a digital photo camera 5D Mark II (Canon, Japan) connected to the microscope with a C-mount adapter (LOMO-MA, Russia). After that the egg was placed on a separate watch glass in tap water which in its turn was placed in a Petri dish. Water was added there regularly as needed. In February 2023 this watch glass was studied in detail with a binocular light microscope MBS-10 (LOMO, USSR), and it was found that the “two-headed” juvenile managed to hatch and was alive, as it demonstrated positive phototaxis. Then the juvenile was caught using a hand-made manipulator consisting of an acupuncture needle 0.16 × 25 mm (Beijing Zhongyan Taihe Medical Instrument Co., China) with a pencil like handle. The juvenile was placed on a microscope slide with a drop of tap water and covered with a coverslip. Another set of light micrographs and videos was taken of intact and heat-killed “two-headed” juvenile using objective lenses with 10×, 20×, 40×, and 100× magnifications (the latter with oil immersion). Morphometry was based on the obtained micrographs using Fiji/ImageJ Version 1.2.4 RRID:SCR_003070 software (National Institutes of Health, USA) in straight line mode (for the egg) and segmented line mode (for the juvenile). The program was set using a microscope calibration slide (transmitted light stage micrometer) OMP (LOMO-MA, Russia).